先恭賀東北大學(xué)武老師使用上海恒遠(yuǎn)生物自主品牌ELISA試劑盒在《ACS Nano》（美國(guó)化學(xué)學(xué)會(huì)）期刊上發(fā)表的SCI文獻(xiàn)——Ratiometric 3D DNA Machine Combined with Machine Learning Algorithm for Ultrasensitive ａnd High-Precision Screening of Early Urinary Diseases順利通過(guò)，IF影響因子高達(dá)15.88分。同時(shí)恭賀武老師喜提上海恒遠(yuǎn)生物贈(zèng)送的獎(jiǎng)學(xué)金，正所謂雙喜臨門(mén)呀！

優(yōu)秀的文獻(xiàn)值得記錄與分享，也希望對(duì)正在做實(shí)驗(yàn)和寫(xiě)論文的科研小伙伴們有所幫助，通過(guò)文獻(xiàn)我們可以更好的了解到產(chǎn)品，如果您也使用恒遠(yuǎn)品牌的ELIA試劑盒發(fā)表了SCI文獻(xiàn)，歡迎您積的參與我們的“發(fā)論文贏獎(jiǎng)學(xué)金”活動(dòng)，小編等著你們的投稿哦，下一個(gè)獎(jiǎng)學(xué)金的說(shuō)不定就是你！服務(wù)熱線：400-0899-208

【文獻(xiàn)標(biāo)題】Ratiometric 3D DNA Machine Combined with Machine Learning Algorithm for Ultrasensitive ａnd High-Precision Screening of Early Urinary Diseases
【作者單位】東北大學(xué)（Northeastern University）
【文獻(xiàn)中引用產(chǎn)品】
人CD63分子（CD63）ELISA試劑盒
【關(guān)鍵詞】uEVs ,DNA machine, fluorescence detection ,machine learning, urinary diseases
【DOI】https://doi.org/10.1021/acsnano.1c06429
【影響因子(IF)】15.88
【出版期刊】《ACS Nano》
【產(chǎn)品原文引用】
Materials.
All the oligonucleotides were synthesized ａnd purified by Shanghai Sangon Biotechnology Co. Ltd. (Shanghai, China), ａnd their sequences are listed in Table S1. All oligonucleotide stock solutions were prepared by dissolving DNA strands in Tris-HCl buffer (10 mmol L-1 Tris, 10 mmol L-1 MgCl2, pH 8.0) ａnd stored at -20 °C refrigerator before use. All the DNA strands were annealed at 95 °C for 5 min ａnd slowly cooled down to room temperature to obtain stable structures. 1-Ethyl-3-[3-(dimethylamino)-propyl] carbodiimide hydrochloride (EDC) ａnd N?hydroxysuccinimide (NHS) were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Sodium hydroxide (NaOH), Na2HPO4·12H2O, NaH2PO4·2H2O, AgNO3 ａnd KCl were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). 2-(N Morpholino) ethanesulfonic acid (MES), Gel-Red, loading buffer ａnd 5×TBE buffer were purchased from Sangon Biotechnology Co. Ltd. (Shanghai, China). BCA Protein Quantification Kit was provided by Beyotime Biotechnology (Shanghai, China). Dulbecco’s modified eagle medium (DMEM) was acquired from Aladdin Reagent Co. Ltd. (Shanghai, China). Carboxyl magnetic nanoparticles (COOH-MNPs, 200 nm) was purchased from PuriMag Biotech Co. Ltd. (Xiamen, China). Exo III, phi29 DNA polymerase were purchased from Sangon Biotechnology Co. Ltd. (Shanghai, China). T4 DNA ligase ａnd deoxynucleotides (dNTPs) were purchased from Takara Biotechnology Co. (Dalian, China). Thioflavine T (ThT) was obtained from Yuanye Bio-Technology Co. Ltd. (Shanghai, China). Anti?CD63 rabbit monoclonal antibody(ab193349) was purchased from Abcam Co. Ltd. (Shang, China). Anti-CD9 rabbit monoclonal antibody (20597-1-ap), anti-TSG101 rabbit monoclonal antibody (28283-1-AP) ａnd β-actin monoclonal antibody (66009-1-Ig) were purchased from Proteintech Group Co. Ltd. (Chicago, USA). ELISA (human CD63) kit was purchased from Shanghai Hengyuan Biological Technology (Shanghai, China). All chemicals were analytical grade ａnd directly used without further purification.

完整版PDF文獻(xiàn)請(qǐng)咨詢(xún)?cè)诰�客服或者電話聯(lián)系我司業(yè)務(wù)員！
更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”微信公眾號(hào)！
或訪問(wèn)恒遠(yuǎn)生物官網(wǎng)（http://www.hnybio.com）

恒遠(yuǎn)生物 業(yè)致力于打造一流的民族品牌，提供全面的、創(chuàng)新的、優(yōu)質(zhì)的、便捷的科研產(chǎn)品和服務(wù)。目，我們自主研發(fā)的重組蛋白、抗體、ELISA檢測(cè)試劑盒得到廣大科研用戶的認(rèn)可，并且多次發(fā)表于權(quán)威期刊，進(jìn)一步推動(dòng)了民族品牌化的傳播。

恒遠(yuǎn)品牌自主研發(fā)的ELISA試劑盒，現(xiàn)已涵蓋20多個(gè)種屬，數(shù)千種產(chǎn)品，涉及腫瘤、激素、自身免疫、心血管、代謝等十多個(gè)研究域。其ELISA試劑盒不僅檢測(cè)指標(biāo)豐富，還可以根據(jù)實(shí)驗(yàn)需求定制檢測(cè)范圍，并且提供實(shí)驗(yàn)外包與免費(fèi)代測(cè)服務(wù)。歡迎您來(lái)電來(lái)函咨詢(xún)。